Last edited by Morg
Thursday, August 6, 2020 | History

6 edition of Immobilisation of DNA on Chips II found in the catalog.

Immobilisation of DNA on Chips II

by Christine Wittmann

  • 79 Want to read
  • 37 Currently reading

Published by Springer .
Written in English

    Subjects:
  • Organic chemistry,
  • Science,
  • Science/Mathematics,
  • Chemistry - Analytic,
  • Chemistry - Inorganic,
  • Chemistry - Organic,
  • Science / Chemistry / Organic,
  • biochemistry,
  • chips,
  • inorganic chemistry,
  • Biosensing Techniques,
  • DNA,
  • DNA microarrays,
  • chemistry

  • Edition Notes

    Topics in Current Chemistry

    The Physical Object
    FormatHardcover
    Number of Pages199
    ID Numbers
    Open LibraryOL9055863M
    ISBN 103540284362
    ISBN 109783540284369

    Immobilisation of DNA on Chips I (Topics in Current Chemistry) (v. 1) [Christine Wittmann] on *FREE* shipping on qualifying offers. M.I. Pividori, S. Alegret: DNA Adsorption on Carbonaceous Materials.- F. Luderer, U. Walschus: Immobilization of Oligonucleotides for Biochemical Sensing by Self-Assembled Monolayers: Thiol-Organic Bonding on Gold and Silanization on Silica . Immobilization of DNA on microarrays / C. Heise, F.F. Bier --Electrochemical adsorption technique for immobilization of single-stranded oligonucleotides onto carbon screen-printed electrodes / I. Palchetti, M. Mascini --DNA immobilization: silanized nucleic acids and nanoprinting / A. Du, O. Larsson, H. Swerdlow, Z. Liang --Immobilization of.

      Recent trends in the development of microfluidic and biodiagnostic chips favor polymer materials over glass, primarly for optical and economical reasons. Therefore, existing chemical methods to prepare biomolecule microarrays on glass slides have to be adapted or replaced in order to suit polymer substrates. Here we present a strategy to immobilize DNA and antibodies on cyclic polyolefin Cited by: In this paper, lipase-immobilized membranes were prepared by both non-covalent and covalent immobilization methods using (i) lipase adsorption on membranes, (ii) inclusion of enzyme in .

      At an immobilization level of RU of the ligand I get a signal of RU of the analyte when using nM of it. At an immobilization level of RU (the only parameter that changes) I . The double-stranded DNA can be chemically denatured, and the chip may be reused with a new analytical sample. Conditions for photopolymerization, hybridization, and denaturation are discussed. We also demonstrate the photopolymerization of plugs containing different DNA probe sequences in one microfluidic channel, thereby enabling the selective.


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Immobilisation of DNA on Chips II by Christine Wittmann Download PDF EPUB FB2

Immobilisation of DNA on Chips II (Topics in Current Chemistry ()) (v. 2) [Wittmann, Christine] on *FREE* shipping on qualifying offers. Immobilisation of DNA on Chips II (Topics in Current Chemistry ()) (v. 2)Format: Hardcover. Immobilisation of DNA on Chips II. Editors; Christine Wittmann; Book.

57 Citations; Immobilisation of DNA on Chips II book Downloads; Part of the Topics in Current Chemistry book series (TOPCURRCHEM, volume ) Chapters Table of contents (8 chapters) About About this book; Table of contents Self-Assembly DNA-Conjugated Polymer for DNA Immobilization on Chip.

Kenji Yokoyama. Immobilisation of DNA on Chips. Books & CD ROMs Show all 2 results. ADD ALL 2 Results TO MARKED ITEMS Hardcover Usually dispatched within 3 to 5 business days.

Immobilisation of DNA on Chips II. Series: Topics in Current Chemistry, Vol. Wittmann, Christine (Ed.) [1] immobilisation of DNA on chips I / C. Wittmann, vol.

editor --[2] immobilisation of DNA on chips II. Series Title: Topics in current chemistry, v. Responsibility: volume editor, Christine Wittmann ; with contributions by S.

Alegret [and others]. More information: Table of contents. Microarrays are new analytical devices that allow the parallel and simultaneous detection of thousands of target compounds. Microarrays, also called DNA chips, are widely used in gene expression, the genotyping of individuals, point mutations, detection of single nucleotide polymorphisms, and short tandem repeats.

We focus the present work on the immobilization of DNA on CMOS compatible materials, which is the first and crucial step for the construction of microarrays. DNA chips were first developed using glass (i.e. glass slides or capillaries), plastic or ceramic by: Our work successfully demonstrates, for the first time, a LSPR microfluidic chip to detect the immobilization of single stranded DNA (ssDNA) mixed with spacer molecules (1-Hexadecanethiol, HDT) on gold nanostructures via thiol-chemistry and subsequently detect their interaction with DNA polymerase enzyme in real-time at room temperature.

Immobilisation of DNA on Chips I It seems that you're in USA. We have a dedicated site Immobilization of Oligonucleotides for Biochemical Sensing by Self-Assembled Monolayers: Thiol-Organic Bonding on Gold and Silanization on Silica Surfaces Book Title Immobilisation of DNA on Chips I Editors.

Christine Wittmann; Series Title Topics in. The immobilization of DNA probes on the working electrode surface to recognize its complementary DNA target via hybridization is the crucial step in the construction of electrochemical DNA sensor.

A good DNA probe immobilization technique would promote a high reactivity and good orientation of immobilized DNA probe to hybridize with its Cited by: Immobilized DNA is required for the development of DNA chips and arrays, DNA sensors, or other sensing devices including microfluidics, in addition to gene delivery devices.

The technology and applications of microarrays of immobilized DNA or oligonucleotides are reviewed. DNA arrays are fabricated by high-speed Cited by: Immobilisation of DNA on Chips I.

Editors; Christine Wittmann Chapters Table of contents (6 chapters) About About this book; Table of contents. Search within book. Front Matter. PDF. DNA Adsorption on Carbonaceous Materials. María Isabel Pividori, Salvador Alegret. Pages Immobilization of Oligonucleotides for Biochemical Sensing by.

Due to this problem, DNA chips with Dendron-modified surfaces need a very high hybridization and washing temperatures and eventually show a very low sensitivity. DNA Immobilization by Using 9G Technology.

The preparation of the 9G DNAChip and the hybridization thereafter is Cited by: After the cleaning step, the sensor chip was covered with a solution (1 μM, 1 ml) of thiolated probe in immobilisation solution (II) and incubated at room temperature for 2 h.

Following incubation, the sensor chip was washed with milliQ water and then it was treated with 1 mM (1 ml) blocking thiol solution (MCH) at room temperature for a Cited by:   Immobilized DNA is required for the development of DNA chips and arrays, DNA sensors, or other sensing devices including microfluidics, in addition to gene delivery devices.

The broad application range for all of these DNA‐based systems is to a major extent found in the medical area, using the devices also in DNA sequencing and furthermore. An efficient heterobifunctional reagent, N-(3-triethoxysilylpropyl)(N′-maleimidylmethyl) cyclohexanamide (TPMC), was developed for the immobilization of thiol-modified oligonucleotides on an unmodified glass heterobifunctionality of the reagent was used for the construction of a DNA microarray in which the triethoxysilyl functionality has specificity toward a glass surface Cited by:   A Pb(II)-specific DNAzyme fluorescent sensor has been modified with a thiol moiety in order to immobilize it on a Au surface.

Self-assembly of the DNAzyme is accomplished by first adsorbing the single-thiolated enzyme strand (HSE-Dy) followed by adsorption of mercaptohexanol, which serves to displace any Au−N interactions and ensure that DNA is bound only through the by: The success of DNA chips depends on the chemistry used for the immobilization of the DNA probes.

Moreover, the success of the DNA chips also depends on the good accessibility and functionality of the surface-bound probes, the density of attachment, and the Cited by: Buy Immobilisation of DNA on Chips: v. 1 (Topics in Current Chemistry) by Wittmann, Christine (ISBN: ) from Amazon's Book Store.

Everyday low prices and free delivery on. By plasma and liquid chemical treatment followed by coating with polyelectrolytes, we have succeeded in immobilizing DNA onto the polymer substrate, yielding stable and versatile biosensor surfaces. We demonstrate the stability and usage of the coated Zeonor substrates not only in DNA chip technology but also in protein chip technology with DNA.

ISBN: OCLC Number: Notes: Chemistry and Materials Science (Springer). Description.item 3 Immobilisation of DNA on Chips II by Q. Du (English) Hardcover Book Free Shippin - Immobilisation of DNA on Chips II by Q.

Du (English) Hardcover Book Free Shippin. $ Free shipping. No ratings or reviews yet. Be the first to write a review. Best Selling in Nonfiction. See all.The immobilisation procedure based on streptavidin–biotin binding, previously optimised with the same DNA probe by this group (Giakoumaki et al.,Mariotti et al., ), was used here as a reference method.

The presence of two flow-cells on the sensor chip allows the immobilisation of two different probes, one for each by: